ADIPOSE TISSUE -HAVING HIGHEST POTENTIAL FOR REGENERATIVE TREATMENT

Now several indications have come up as evidence-based treatments backed by years of research and have been published in many peer-reviewed indexed journals.

This forum has found a reliable source of information on Adipose-derived regenerative treatments. Check it out today!


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Level of

Evidences

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Find the latest research and insights on several indications on this forum.

CELL COUNT

Richest Source of Regenerative Cells

ISOLATION

Can be done with Mechanical, Enzyme or Culture

AGE IMPACT

No decline in cell count with age as compared to bone marrow

Fat is not a tissue it is a Complex organ.

Adipose is a highly vascularized structure, composed of a heterogeneous mixture of cell populations, primarily derived from interlobular and perivascular connective tissues, consisting of mature adipocytes, preadipocytes, fibroblasts, vascular smooth muscle cells, endothelial cells, resident monocytes/macrophages, and lymphocytes, as well as progenitor cells and pericytes /stromal cells.

3

Reasons

Why regenerative expert loves your fat you might hate?

In terms of regenerative cells, fat is one of the richest sources

Autologous fat grafting

Is A safe and effective method with no allergy, no rejection, ease of harvesting and administration.

Fat contains

Fat contains a heterogeneous combination of cells such aspericytes, adipocytes, MSCs, endothelial progenitor cells, M2 macrophages, Bcell, T cells, mast cell, extracellular matrix, and others which has shown the remarkable capability of regeneration, immunomodulation, and cure in several indications.

Isolation Technique

Just you need to identify the Right isolation technique that uses Minimal Manipulation.

adipose

Adipose vs Bone marrow

Adipose Stromal Vascular Fraction (SVF) vs Bone Marrow Concentrate (BMC)

Parameter

Bone Marrow

Stromal Vascular
Fraction (SVF)
Major Type of cells present Hematopoietic cells Heterogenous combination of cells
Cell count with Age Decrease with age Doesn’t decrease because renewable growth of fat
Total No. of cells Comparatively less 100 to 1000’s time more than bone marrow
Use of Enzymes/ Culture Is required to get sufficient number of cells Not required with SVF technology with minimal manipulation
Process Painful & invasive Less painful & minimally invasive

Adipose(SVF) vs PRP

PRP vs Adipose Stromal Vascular Fraction (SVF)

Parameter

Platelet rich
plasma

Stromal Vascular
Fraction (SVF)
Source Blood Adipose tissue
Contain Growth factors & cytokines Heterogeneous mixture of cells
Effect Lasting Short lasting effect More than 5 years*
Mechanism of action Doesn’t directly replace damaged cells
  • Differentiation
  • Homing effect
  • Anti inflammatory
  • Angiogenesis
  • Secretion of cytokines
  • Extra Cellular Matrix
    		•

    Enzymatic vs Mechanical

    Enzymatic vs Mechanical

    Parameter

    Enzymatic Method

    Mechanical method
    No. of surgical sitting More than one sitting required Done in same surgical sitting
    Additives used Collagenase/ lysin etc No additives used
    Extra cellular matrix Not present Present which act as a scaffold
    Time required for harvesting Less More
    Level of manipulation More than minimal manipulation Minimal manipulation
    Regulatory clearance Not having clearance Clearance from regulatory

    Direct vs Indirect Sonication

    Direct vs Indirect Sonication

    Parameter

    Direct Sonication

    Indirect Sonicaltion
    No. of surgical sitting More than one sitting required Done in same surgical sitting
    Energy Transfer Directly in adipose tissue through a probe or sonicator Energy transferred through a water bath no direct contact of sample
    Intensity of sonic wave Less Higher
    Processing time Less Higher
    Cell Count Higher cell count Lower Cell count
    Viability Higher viable cells Less viable cells
    Sample volume Can be used of any volume of sample Recommended for small volume of sample
    Reference image